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Retron-mediated multiplex genome editing and continuous evolution in Escherichia coli
While there are several genome editing techniques available, few are suitable for dynamic and simultaneous mutagenesis of arbitrary targeted sequences in prokaryotes. Here, to address these limitations, we present a versatile and multiplex retron-mediated genome editing system (REGES). First, throug...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450171/ https://www.ncbi.nlm.nih.gov/pubmed/37471041 http://dx.doi.org/10.1093/nar/gkad607 |