Detection and Quantification of Calcium Ions in the Endoplasmic Reticulum and Cytoplasm of Cultured Cells Using Fluorescent Reporter Proteins and ImageJ Software

This protocol describes a method for detecting and quantifying calcium ions in the endoplasmic reticulum (ER) and cytoplasm of cultured cells using fluorescent reporter proteins and ImageJ software. Genetically engineered fluorescent reporter proteins, such as G-CEPIA1er and GCaMP6f, localize to int...

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Detalles Bibliográficos
Autores principales: Saito, Shunsuke, Mori, Kazutoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450730/
https://www.ncbi.nlm.nih.gov/pubmed/37638301
http://dx.doi.org/10.21769/BioProtoc.4738

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450730/
https://www.ncbi.nlm.nih.gov/pubmed/37638301
http://dx.doi.org/10.21769/BioProtoc.4738

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