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Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells

Olive tree by-products have been deeply studied as an invaluable source of bioactive compounds. Several in vitro and in vivo studies showed that olive leaf extract (OLE) has anti-inflammatory and antioxidant properties. Here, we wanted to assess the valuable benefits of two less-studied OLE componen...

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Autores principales: Silvestrini, Andrea, Giordani, Chiara, Bonacci, Sonia, Giuliani, Angelica, Ramini, Deborah, Matacchione, Giulia, Sabbatinelli, Jacopo, Di Valerio, Silvia, Pacetti, Deborah, Procopio, Antonio Domenico, Procopio, Antonio, Rippo, Maria Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451521/
https://www.ncbi.nlm.nih.gov/pubmed/37627504
http://dx.doi.org/10.3390/antiox12081509
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author Silvestrini, Andrea
Giordani, Chiara
Bonacci, Sonia
Giuliani, Angelica
Ramini, Deborah
Matacchione, Giulia
Sabbatinelli, Jacopo
Di Valerio, Silvia
Pacetti, Deborah
Procopio, Antonio Domenico
Procopio, Antonio
Rippo, Maria Rita
author_facet Silvestrini, Andrea
Giordani, Chiara
Bonacci, Sonia
Giuliani, Angelica
Ramini, Deborah
Matacchione, Giulia
Sabbatinelli, Jacopo
Di Valerio, Silvia
Pacetti, Deborah
Procopio, Antonio Domenico
Procopio, Antonio
Rippo, Maria Rita
author_sort Silvestrini, Andrea
collection PubMed
description Olive tree by-products have been deeply studied as an invaluable source of bioactive compounds. Several in vitro and in vivo studies showed that olive leaf extract (OLE) has anti-inflammatory and antioxidant properties. Here, we wanted to assess the valuable benefits of two less-studied OLE components—3,4-DHPEA-EDA (Oleacin, OC) and 3,4-DHPEA-EA (Oleuropein-Aglycone, OA)—directly purified from OLE using a cost-effective and environmentally sustainable method, in line with the principles of circular economy. OLE, OC and OA were then tested in human cellular models involved in acute and chronic inflammation and in the pathogenesis of viral infections, i.e., lipopolysaccharide (LPS)-treated monocyte/macrophages (THP-1) and endothelial cells (HUVECs), senescent HUVECs and Poly(I:C)-treated small airway epithelial cells (hSAECs). Results showed that OC and OA are efficient in ameliorating almost all of the pro-inflammatory readouts (IL-1β, TNF-α, IL-8, ICAM, VCAM) and reducing the release of IL-6 in all the cellular models. In hSAECs, they also modulate the expression of SOD2, NF-kB and also ACE2 and TMPRSS2, whose expression is required for SARS-CoV-2 virus entry. Overall, these data suggest the usefulness of OLE, OC and OA in controlling or preventing inflammatory responses, in particular those associated with viral respiratory infections and aging.
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spelling pubmed-104515212023-08-26 Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells Silvestrini, Andrea Giordani, Chiara Bonacci, Sonia Giuliani, Angelica Ramini, Deborah Matacchione, Giulia Sabbatinelli, Jacopo Di Valerio, Silvia Pacetti, Deborah Procopio, Antonio Domenico Procopio, Antonio Rippo, Maria Rita Antioxidants (Basel) Article Olive tree by-products have been deeply studied as an invaluable source of bioactive compounds. Several in vitro and in vivo studies showed that olive leaf extract (OLE) has anti-inflammatory and antioxidant properties. Here, we wanted to assess the valuable benefits of two less-studied OLE components—3,4-DHPEA-EDA (Oleacin, OC) and 3,4-DHPEA-EA (Oleuropein-Aglycone, OA)—directly purified from OLE using a cost-effective and environmentally sustainable method, in line with the principles of circular economy. OLE, OC and OA were then tested in human cellular models involved in acute and chronic inflammation and in the pathogenesis of viral infections, i.e., lipopolysaccharide (LPS)-treated monocyte/macrophages (THP-1) and endothelial cells (HUVECs), senescent HUVECs and Poly(I:C)-treated small airway epithelial cells (hSAECs). Results showed that OC and OA are efficient in ameliorating almost all of the pro-inflammatory readouts (IL-1β, TNF-α, IL-8, ICAM, VCAM) and reducing the release of IL-6 in all the cellular models. In hSAECs, they also modulate the expression of SOD2, NF-kB and also ACE2 and TMPRSS2, whose expression is required for SARS-CoV-2 virus entry. Overall, these data suggest the usefulness of OLE, OC and OA in controlling or preventing inflammatory responses, in particular those associated with viral respiratory infections and aging. MDPI 2023-07-28 /pmc/articles/PMC10451521/ /pubmed/37627504 http://dx.doi.org/10.3390/antiox12081509 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silvestrini, Andrea
Giordani, Chiara
Bonacci, Sonia
Giuliani, Angelica
Ramini, Deborah
Matacchione, Giulia
Sabbatinelli, Jacopo
Di Valerio, Silvia
Pacetti, Deborah
Procopio, Antonio Domenico
Procopio, Antonio
Rippo, Maria Rita
Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells
title Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells
title_full Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells
title_fullStr Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells
title_full_unstemmed Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells
title_short Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells
title_sort anti-inflammatory effects of olive leaf extract and its bioactive compounds oleacin and oleuropein-aglycone on senescent endothelial and small airway epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10451521/
https://www.ncbi.nlm.nih.gov/pubmed/37627504
http://dx.doi.org/10.3390/antiox12081509
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