Improving the Thermostability of Serine Protease PB92 from Bacillus alcalophilus via Site-Directed Mutagenesis Based on Semi-Rational Design

Proteases have been widely employed in many industrial processes. In this work, we aimed to improve the thermostability of the serine protease PB92 from Bacillus alcalophilus to meet the high-temperature requirements of biotechnological treatments. Eight mutation sites (N18, S97-S101, E110, and R143...

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Detalles Bibliográficos
Autores principales: Miao, Huabiao, Xiang, Xia, Han, Nanyu, Wu, Qian, Huang, Zunxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453622/
https://www.ncbi.nlm.nih.gov/pubmed/37628080
http://dx.doi.org/10.3390/foods12163081

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10453622/
https://www.ncbi.nlm.nih.gov/pubmed/37628080
http://dx.doi.org/10.3390/foods12163081

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