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Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites

The assembly of α-synuclein into cross-β structured amyloid fibers results in Lewy body deposits and neuronal degeneration in Parkinson’s disease patients. As the cell environment is highly crowded, interactions between the formed amyloid fibers and a range of biomolecules can occur in cells. Althou...

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Autores principales: Horvath, Istvan, Mohamed, Khadra A., Kumar, Ranjeet, Wittung-Stafshede, Pernilla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454467/
https://www.ncbi.nlm.nih.gov/pubmed/37629028
http://dx.doi.org/10.3390/ijms241612849
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author Horvath, Istvan
Mohamed, Khadra A.
Kumar, Ranjeet
Wittung-Stafshede, Pernilla
author_facet Horvath, Istvan
Mohamed, Khadra A.
Kumar, Ranjeet
Wittung-Stafshede, Pernilla
author_sort Horvath, Istvan
collection PubMed
description The assembly of α-synuclein into cross-β structured amyloid fibers results in Lewy body deposits and neuronal degeneration in Parkinson’s disease patients. As the cell environment is highly crowded, interactions between the formed amyloid fibers and a range of biomolecules can occur in cells. Although amyloid fibers are considered chemically inert species, recent in vitro work using model substrates has shown α-synuclein amyloids, but not monomers, to catalyze the hydrolysis of ester and phosphoester bonds. To search for putative catalytic activity of α-synuclein amyloids on biologically relevant metabolites, we here incubated α-synuclein amyloids with neuronal SH-SY5Y cell lysates devoid of proteins. LC-MS-based metabolomic (principal component and univariate) analysis unraveled distinct changes in several metabolite levels upon amyloid (but not monomer) incubation. Of 63 metabolites identified, the amounts of four increased (3-hydroxycapric acid, 2-pyrocatechuic acid, adenosine, and NAD), and the amounts of seventeen decreased (including aromatic and apolar amino acids, metabolites in the TCA cycle, keto acids) in the presence of α-synuclein amyloids. Many of these metabolite changes match what has been reported previously in Parkinson’s disease patients and animal–model metabolomics studies. Chemical reactivity of α-synuclein amyloids may be a new gain-of-function that alters the metabolite composition in cells and, thereby, modulates disease progression.
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spelling pubmed-104544672023-08-26 Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites Horvath, Istvan Mohamed, Khadra A. Kumar, Ranjeet Wittung-Stafshede, Pernilla Int J Mol Sci Communication The assembly of α-synuclein into cross-β structured amyloid fibers results in Lewy body deposits and neuronal degeneration in Parkinson’s disease patients. As the cell environment is highly crowded, interactions between the formed amyloid fibers and a range of biomolecules can occur in cells. Although amyloid fibers are considered chemically inert species, recent in vitro work using model substrates has shown α-synuclein amyloids, but not monomers, to catalyze the hydrolysis of ester and phosphoester bonds. To search for putative catalytic activity of α-synuclein amyloids on biologically relevant metabolites, we here incubated α-synuclein amyloids with neuronal SH-SY5Y cell lysates devoid of proteins. LC-MS-based metabolomic (principal component and univariate) analysis unraveled distinct changes in several metabolite levels upon amyloid (but not monomer) incubation. Of 63 metabolites identified, the amounts of four increased (3-hydroxycapric acid, 2-pyrocatechuic acid, adenosine, and NAD), and the amounts of seventeen decreased (including aromatic and apolar amino acids, metabolites in the TCA cycle, keto acids) in the presence of α-synuclein amyloids. Many of these metabolite changes match what has been reported previously in Parkinson’s disease patients and animal–model metabolomics studies. Chemical reactivity of α-synuclein amyloids may be a new gain-of-function that alters the metabolite composition in cells and, thereby, modulates disease progression. MDPI 2023-08-16 /pmc/articles/PMC10454467/ /pubmed/37629028 http://dx.doi.org/10.3390/ijms241612849 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Horvath, Istvan
Mohamed, Khadra A.
Kumar, Ranjeet
Wittung-Stafshede, Pernilla
Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
title Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
title_full Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
title_fullStr Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
title_full_unstemmed Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
title_short Amyloids of α-Synuclein Promote Chemical Transformations of Neuronal Cell Metabolites
title_sort amyloids of α-synuclein promote chemical transformations of neuronal cell metabolites
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454467/
https://www.ncbi.nlm.nih.gov/pubmed/37629028
http://dx.doi.org/10.3390/ijms241612849
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