In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study

In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population...

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Autores principales: Seisenov, Bolat, Duimbayev, Dulat, Kazhgaliyev, Nurlybay, Abdrakhmanov, Talgat, Tegza, Alexandra, Abeldinov, Rustem, Burambayeva, Nadezhda, Temirzhanova, Alma, Tegza, Ivan, Kemeshev, Zhomart, Zhanabayev, Assylbek, Akhmetbekov, Nurbolat, Aisin, Marat, Zhugunissov, Kuandyk, Issimov, Arman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456122/
https://www.ncbi.nlm.nih.gov/pubmed/37629490
http://dx.doi.org/10.3390/life13081632
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author Seisenov, Bolat
Duimbayev, Dulat
Kazhgaliyev, Nurlybay
Abdrakhmanov, Talgat
Tegza, Alexandra
Abeldinov, Rustem
Burambayeva, Nadezhda
Temirzhanova, Alma
Tegza, Ivan
Kemeshev, Zhomart
Zhanabayev, Assylbek
Akhmetbekov, Nurbolat
Aisin, Marat
Zhugunissov, Kuandyk
Issimov, Arman
author_facet Seisenov, Bolat
Duimbayev, Dulat
Kazhgaliyev, Nurlybay
Abdrakhmanov, Talgat
Tegza, Alexandra
Abeldinov, Rustem
Burambayeva, Nadezhda
Temirzhanova, Alma
Tegza, Ivan
Kemeshev, Zhomart
Zhanabayev, Assylbek
Akhmetbekov, Nurbolat
Aisin, Marat
Zhugunissov, Kuandyk
Issimov, Arman
author_sort Seisenov, Bolat
collection PubMed
description In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (p ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms.
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spelling pubmed-104561222023-08-26 In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study Seisenov, Bolat Duimbayev, Dulat Kazhgaliyev, Nurlybay Abdrakhmanov, Talgat Tegza, Alexandra Abeldinov, Rustem Burambayeva, Nadezhda Temirzhanova, Alma Tegza, Ivan Kemeshev, Zhomart Zhanabayev, Assylbek Akhmetbekov, Nurbolat Aisin, Marat Zhugunissov, Kuandyk Issimov, Arman Life (Basel) Communication In vitro fertilization (IVF) technologies have great potential in the preservation of endangered species. In the current study, an IVF experiment was carried out to evaluate whether reproductive technologies are suitable for Kazakh Whiteheaded cattle, aimed at preserving this breed whose population has reduced drastically over the last thirty years. The reproduction characteristics of Kazakh Whiteheaded cows were compared to Aberdeen Angus cows. Transvaginal ultrasound-guided ovum pick up sessions were carried out followed by in vitro embryo production and embryo transfer and pregnancy diagnosis. The total and viable oocytes per OPU procedure were 12.8 ± 1.18 and 8.7 ± 0.85 for the Aberdeen Angus breed, and 8.8 ± 1.04 and 6.2 ± 0.83 for the Kazakh Whiteheaded breed. Similarly, the mean number of cleaved oocytes and morula/blastocyst stage embryos produced by OPU/IVF were 4.8 ± 0.49 and 1.4 ± 0.15 for the Aberdeen Angus breed, and 2.4 ± 0.46 and 0.18 ± 0.05 for the Kazakh Whiteheaded breed (p ≤ 0.02). From fifty Kazakh Whiteheaded donor animals, 2585 oocytes were aspirated following six ovum pick up sessions. One thousand eight hundred and seventy-six (72.5%) oocytes were chosen for maturation and were further fertilized. The number of embryos cleaved was 720 (38.3% out of oocytes fertilized) on day four post-fertilization. Of these cleaved embryos, 56 (7.5%) developed into the late morula/blastocyst stage on day seven post-fertilization, averaging 1.12 embryos per donor animal. Pregnancy was detected in 12 recipients; 4 healthy calves have been born to date. The outcomes of our study have demonstrated that reproductive technologies can be applicable in preserving the endangered Kazakh Whiteheaded cattle. The findings in this report will enhance knowledge of the reproductive characteristics of endangered domestic animals and help develop sophisticated reproductive protocols for animals with unique reproductive mechanisms. MDPI 2023-07-27 /pmc/articles/PMC10456122/ /pubmed/37629490 http://dx.doi.org/10.3390/life13081632 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Seisenov, Bolat
Duimbayev, Dulat
Kazhgaliyev, Nurlybay
Abdrakhmanov, Talgat
Tegza, Alexandra
Abeldinov, Rustem
Burambayeva, Nadezhda
Temirzhanova, Alma
Tegza, Ivan
Kemeshev, Zhomart
Zhanabayev, Assylbek
Akhmetbekov, Nurbolat
Aisin, Marat
Zhugunissov, Kuandyk
Issimov, Arman
In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
title In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
title_full In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
title_fullStr In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
title_full_unstemmed In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
title_short In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study
title_sort in vitro fertilization in kazakh whiteheaded cattle: a comparative study
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10456122/
https://www.ncbi.nlm.nih.gov/pubmed/37629490
http://dx.doi.org/10.3390/life13081632
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