TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method

Molecular cloning is used in a wide variety of biological and medical research. Here, we developed a rapid and efficient DNA-assembling method for routine laboratory work. We discovered that the cleavage speed of T5 exonuclease is approximately 3 nt/min at 0°C and hence developed a T5 exonuclease–me...

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Detalles Bibliográficos
Autores principales: Yu, Fang, Li, Xia, Wang, Fei, Liu, Yang, Zhai, Chao, Li, Wenqiang, Ma, Lixin, Chen, Wanping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457141/
https://www.ncbi.nlm.nih.gov/pubmed/37635997
http://dx.doi.org/10.3389/fbioe.2023.1167534

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457141/
https://www.ncbi.nlm.nih.gov/pubmed/37635997
http://dx.doi.org/10.3389/fbioe.2023.1167534

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