Quantification of membrane-bound cytokine receptors by calibrated flow cytometry

We present a protocol for quantifying the expression of the receptor gp130 using a calibrated flow cytometric approach. We describe pitfalls for receptor quantification such as titration of primary antibodies and standardizing cell culture. Receptors are stained with primary antibodies and fluoropho...

Descripción completa

Detalles Bibliográficos
Autores principales: Miri, Niloufarsadat, Köhler, Nadine, Dittrich, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457439/
https://www.ncbi.nlm.nih.gov/pubmed/37581983
http://dx.doi.org/10.1016/j.xpro.2023.102511

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457439/
https://www.ncbi.nlm.nih.gov/pubmed/37581983
http://dx.doi.org/10.1016/j.xpro.2023.102511

Ejemplares similares