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Quantification of membrane-bound cytokine receptors by calibrated flow cytometry
We present a protocol for quantifying the expression of the receptor gp130 using a calibrated flow cytometric approach. We describe pitfalls for receptor quantification such as titration of primary antibodies and standardizing cell culture. Receptors are stained with primary antibodies and fluoropho...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457439/ https://www.ncbi.nlm.nih.gov/pubmed/37581983 http://dx.doi.org/10.1016/j.xpro.2023.102511 |