Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe

Schizosaccharomyces pombe Dim1 is a conserved essential component of the U4/U6.U5 tri-snRNP complex essential for pre-mRNA splicing. In a synthetic lethal screen with the temperature-sensitive dim1-35 mutant, we isolated multiple alleles of non-essential mtl16 that encodes the U6 snRNA m (6) A methy...

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Autores principales: Willet, Alaina H., Ren, Liping, Turner, Lesley A., Gould, Kathleen L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Findings Previously Not Shown
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457676/
https://www.ncbi.nlm.nih.gov/pubmed/37637271
http://dx.doi.org/10.17912/micropub.biology.000948
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author Willet, Alaina H.
Ren, Liping
Turner, Lesley A.
Gould, Kathleen L.
author_facet Willet, Alaina H.
Ren, Liping
Turner, Lesley A.
Gould, Kathleen L.
author_sort Willet, Alaina H.
collection PubMed
description Schizosaccharomyces pombe Dim1 is a conserved essential component of the U4/U6.U5 tri-snRNP complex essential for pre-mRNA splicing. In a synthetic lethal screen with the temperature-sensitive dim1-35 mutant, we isolated multiple alleles of non-essential mtl16 that encodes the U6 snRNA m (6) A methyltransferase. Further genetic analysis revealed strong and specific negative genetic interactions between mtl16 and a mutation in the Dim1 binding partner, Prp31, and between dim1-35 and a mutation in the Prp31 binding partner, Prp6. Our work provides additional tools to study pre-mRNA splicing in S. pombe and biological confirmation of the importance of the Prp6-Prp31-Dim1-U6 snRNA interactions for pre-mRNA splicing.
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spelling pubmed-104576762023-08-27 Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe Willet, Alaina H. Ren, Liping Turner, Lesley A. Gould, Kathleen L. MicroPubl Biol Findings Previously Not Shown Schizosaccharomyces pombe Dim1 is a conserved essential component of the U4/U6.U5 tri-snRNP complex essential for pre-mRNA splicing. In a synthetic lethal screen with the temperature-sensitive dim1-35 mutant, we isolated multiple alleles of non-essential mtl16 that encodes the U6 snRNA m (6) A methyltransferase. Further genetic analysis revealed strong and specific negative genetic interactions between mtl16 and a mutation in the Dim1 binding partner, Prp31, and between dim1-35 and a mutation in the Prp31 binding partner, Prp6. Our work provides additional tools to study pre-mRNA splicing in S. pombe and biological confirmation of the importance of the Prp6-Prp31-Dim1-U6 snRNA interactions for pre-mRNA splicing. Caltech Library 2023-08-11 /pmc/articles/PMC10457676/ /pubmed/37637271 http://dx.doi.org/10.17912/micropub.biology.000948 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Findings Previously Not Shown
Willet, Alaina H.
Ren, Liping
Turner, Lesley A.
Gould, Kathleen L.
Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe
title Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe
title_full Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe
title_fullStr Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe
title_full_unstemmed Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe
title_short Isolation of mutant alleles of the U6 snRNA m (6) A methyltransferase Mtl16 and characterization of their genetic interactions with splicing mutants in Schizosaccharomyces pombe
title_sort isolation of mutant alleles of the u6 snrna m (6) a methyltransferase mtl16 and characterization of their genetic interactions with splicing mutants in schizosaccharomyces pombe
topic Findings Previously Not Shown
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10457676/
https://www.ncbi.nlm.nih.gov/pubmed/37637271
http://dx.doi.org/10.17912/micropub.biology.000948
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