Quantitative cross-linking via engineered cysteines to study inter-domain interactions in bacterial collagenases

Inter-domain movements act as important activity modulators in multi-domain proteins. Here, we present a protocol for inter-domain cross-linking via engineered cysteines. Using collagenase G (ColG) from Hathewaya histolytica as a model, we describe steps for the design, expression, purification, and...

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Detalles Bibliográficos
Autores principales: Serwanja, Jamil, Brandstetter, Hans, Schönauer, Esther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458335/
https://www.ncbi.nlm.nih.gov/pubmed/37605531
http://dx.doi.org/10.1016/j.xpro.2023.102519

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458335/
https://www.ncbi.nlm.nih.gov/pubmed/37605531
http://dx.doi.org/10.1016/j.xpro.2023.102519

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