A Novel Strategy of US3 Codon De-Optimization for Construction of an Attenuated Pseudorabies Virus against High Virulent Chinese Pseudorabies Virus Variant

In this study, we applied bacterial artificial chromosome (BAC) technology with PRV(ΔTK/gE/gI) as the base material to replace the first, central, and terminal segments of the US3 gene with codon-deoptimized fragments via two-step Red-mediated recombination in E. coli GS1783 cells. The three constru...

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Detalles Bibliográficos
Autores principales: Xu, Mengwei, Wang, Yiwei, Liu, Yamei, Chen, Saisai, Zhu, Laixu, Tong, Ling, Zheng, Yating, Osterrieder, Nikolaus, Zhang, Chuanjian, Wang, Jichun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458909/
https://www.ncbi.nlm.nih.gov/pubmed/37631856
http://dx.doi.org/10.3390/vaccines11081288

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10458909/
https://www.ncbi.nlm.nih.gov/pubmed/37631856
http://dx.doi.org/10.3390/vaccines11081288

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