A novel binding site between the voltage-dependent calcium channel Ca(V)1.2 subunit and Ca(V)β2 subunit discovered using a new analysis method for protein–protein interactions

We developed a new method to analyze protein–protein interactions using a dual-inducible prokaryotic expression system. To evaluate protein–protein binding, a chimeric fusion toxin gene was constructed using a DNase-treated short DNA fragment (epitope library) and CcdB, which encodes a DNA topoisome...

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Detalles Bibliográficos
Autores principales: Murakami, Agnieszka M., Nagatomo, Katsuhiro, Miyoshi, Ichro, Itagaki, Shirou, Niwa, Yasutaka, Murakami, Manabu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460381/
https://www.ncbi.nlm.nih.gov/pubmed/37634019
http://dx.doi.org/10.1038/s41598-023-41168-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460381/
https://www.ncbi.nlm.nih.gov/pubmed/37634019
http://dx.doi.org/10.1038/s41598-023-41168-4

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