Interstrand crosslinking of homologous repair template DNA enhances gene editing in human cells

We describe a strategy to boost the efficiency of gene editing via homology-directed repair (HDR) by covalently modifying the template DNA with interstrand crosslinks. Crosslinked templates (xHDRTs) increase Cas9-mediated editing efficiencies by up to fivefold in K562, HEK293T, U2OS, iPS and primary...

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Detalles Bibliográficos
Autores principales: Ghasemi, Hannah I., Bacal, Julien, Yoon, Amanda C., Tavasoli, Katherine U., Cruz, Carmen, Vu, Jonathan T., Gardner, Brooke M., Richardson, Chris D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460463/
https://www.ncbi.nlm.nih.gov/pubmed/36849829
http://dx.doi.org/10.1038/s41587-022-01654-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460463/
https://www.ncbi.nlm.nih.gov/pubmed/36849829
http://dx.doi.org/10.1038/s41587-022-01654-y

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