Enhancing CRISPR prime editing by reducing misfolded pegRNA interactions

CRISPR prime editing (PE) requires a Cas9 nickase-reverse transcriptase fusion protein (known as PE2) and a prime editing guide RNA (pegRNA), an extended version of a standard guide RNA (gRNA) that both specifies the intended target genomic sequence and encodes the desired genetic edit. Here we show...

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Detalles Bibliográficos
Autores principales: Zhang, Weiting, Petri, Karl, Ma, Junyan, Lee, Hyunho, Tsai, Chia-Lun, Joung, J. Keith, Yeh, Jing-Ruey Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462064/
https://www.ncbi.nlm.nih.gov/pubmed/37645936
http://dx.doi.org/10.1101/2023.08.14.553324

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462064/
https://www.ncbi.nlm.nih.gov/pubmed/37645936
http://dx.doi.org/10.1101/2023.08.14.553324

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