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Histological analysis of invasive glioblastoma organoids embedded in a 3D collagen matrix
Organoids are unique tools to mimic how tumors evolve in a 3D environment. Here, we present a protocol to embed spheroids invading a 3D matrix into a paraffin mold. We describe steps for preparing spheroids, collagen and agarose inclusion, and paraffinization. We then detail procedures for sectionin...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10462880/ https://www.ncbi.nlm.nih.gov/pubmed/37597188 http://dx.doi.org/10.1016/j.xpro.2023.102521 |