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Minimal resin embedding of SBF-SEM samples reduces charging and facilitates finding a surface-linked region of interest

BACKGROUND: For decoding the mechanism of how cells and organs function information on their ultrastructure is essential. High-resolution 3D imaging has revolutionized morphology. Serial block face scanning electron microscopy (SBF-SEM) offers non-laborious, automated imaging in 3D of up to ~ 1 mm(3...

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Detalles Bibliográficos
Autores principales: Konopová, Barbora, Týč, Jiří
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10463905/
https://www.ncbi.nlm.nih.gov/pubmed/37641135
http://dx.doi.org/10.1186/s12983-023-00507-x