Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study

Timely detection of outbreaks is needed for poliovirus eradication, but gold standard detection in the Democratic Republic of the Congo takes 30 days (median). Direct molecular detection and nanopore sequencing (DDNS) of poliovirus in stool samples is a promising fast method. Here we report prospect...

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Detalles Bibliográficos
Autores principales: Shaw, Alexander G., Mampuela, Tresor Kabeya, Lofiko, Emmanuel Lokilo, Pratt, Catherine, Troman, Catherine, Bujaki, Erika, O’Toole, Áine, Akello, Joyce Odeke, Aziza, Adrienne Amuri, Lusamaki, Eddy Kinganda, Makangara, Jean Claude, Akonga, Marceline, Lay, Yvonne, Nsunda, Bibiche, White, Bailey, Jorgensen, David, Pukuta, Elizabeth, Riziki, Yogolelo, Rankin, Kathleen E., Rambaut, Andrew, Ahuka-Mundeke, Steve, Muyembe, Jean-Jacques, Martin, Javier, Grassly, Nicholas C., Mbala-Kingebeni, Placide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465353/
https://www.ncbi.nlm.nih.gov/pubmed/37591995
http://dx.doi.org/10.1038/s41564-023-01453-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10465353/
https://www.ncbi.nlm.nih.gov/pubmed/37591995
http://dx.doi.org/10.1038/s41564-023-01453-4

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