A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli

Molecular cloning and controlled expression remain challenging when the target gene encodes a protein that is toxic to the host. We developed a set of multi-layer control systems to enable cloning of genes encoding proteins known to be highly toxic in Escherichia coli and other bacteria. The differe...

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Detalles Bibliográficos
Autores principales: Vandierendonck, Jessie, Girardin, Yana, De Bruyn, Pieter, De Greve, Henri, Loris, Remy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10467106/
https://www.ncbi.nlm.nih.gov/pubmed/37624265
http://dx.doi.org/10.3390/toxins15080508

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10467106/
https://www.ncbi.nlm.nih.gov/pubmed/37624265
http://dx.doi.org/10.3390/toxins15080508

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