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A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila
CRISPR nucleases generate a broad spectrum of mutations that includes undesired editing outcomes. Here, we develop optimized C-to-T base editing systems for the generation of precise loss- or gain-of-function alleles in Drosophila and identify temperature as a crucial parameter for efficiency. We fi...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Association for the Advancement of Science
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468138/ https://www.ncbi.nlm.nih.gov/pubmed/37647411 http://dx.doi.org/10.1126/sciadv.adj1568 |