Cargando…

Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seq

Ribosome profiling (Ribo-seq) measures ribosome density along messenger RNA (mRNA) transcripts and is used to estimate the “translational fitness” of a given mRNA in response to environmental or developmental cues with high resolution. Here, we describe a protocol for Ribo-seq in plants adapted for...

Descripción completa

Detalles Bibliográficos
Autores principales: Vélez-Bermúdez, Isabel Cristina, Chou, Shu-Jen, Chen, Ai-Ping, Lin, Wen-Dar, Schmidt, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469065/
https://www.ncbi.nlm.nih.gov/pubmed/37597190
http://dx.doi.org/10.1016/j.xpro.2023.102520