Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9

CRISPR/Cas9 genome editing technology has been implemented in almost all living organisms. Its editing precision appears to be very high and therefore could represent a big change from conventional genetic engineering approaches. However, guide RNA binding to nucleotides similar to the target site c...

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Detalles Bibliográficos
Autores principales: Garrigues, Sandra, Peng, Mao, Kun, Roland S., de Vries, Ronald P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470509/
https://www.ncbi.nlm.nih.gov/pubmed/37486124
http://dx.doi.org/10.1128/mbio.00668-23

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10470509/
https://www.ncbi.nlm.nih.gov/pubmed/37486124
http://dx.doi.org/10.1128/mbio.00668-23

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