Purinergic signaling mediates neuroglial interactions to modulate sighs

Sighs prevent the collapse of alveoli in the lungs, initiate arousal under hypoxic conditions, and are an expression of sadness and relief. Sighs are periodically superimposed on normal breaths, known as eupnea. Implicated in the generation of these rhythmic behaviors is the preBötzinger complex (pr...

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Autores principales: Severs, Liza J., Bush, Nicholas E., Quina, Lely A., Hidalgo-Andrade, Skyler, Burgraff, Nicholas J., Dashevskiy, Tatiana, Shih, Andy Y., Baertsch, Nathan A., Ramirez, Jan-Marino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10471608/
https://www.ncbi.nlm.nih.gov/pubmed/37652903
http://dx.doi.org/10.1038/s41467-023-40812-x
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author Severs, Liza J.
Bush, Nicholas E.
Quina, Lely A.
Hidalgo-Andrade, Skyler
Burgraff, Nicholas J.
Dashevskiy, Tatiana
Shih, Andy Y.
Baertsch, Nathan A.
Ramirez, Jan-Marino
author_facet Severs, Liza J.
Bush, Nicholas E.
Quina, Lely A.
Hidalgo-Andrade, Skyler
Burgraff, Nicholas J.
Dashevskiy, Tatiana
Shih, Andy Y.
Baertsch, Nathan A.
Ramirez, Jan-Marino
author_sort Severs, Liza J.
collection PubMed
description Sighs prevent the collapse of alveoli in the lungs, initiate arousal under hypoxic conditions, and are an expression of sadness and relief. Sighs are periodically superimposed on normal breaths, known as eupnea. Implicated in the generation of these rhythmic behaviors is the preBötzinger complex (preBötC). Our experimental evidence suggests that purinergic signaling is necessary to generate spontaneous and hypoxia-induced sighs in a mouse model. Our results demonstrate that driving calcium increases in astrocytes through pharmacological methods robustly increases sigh, but not eupnea, frequency. Calcium imaging of preBötC slices corroborates this finding with an increase in astrocytic calcium upon application of sigh modulators, increasing intracellular calcium through g-protein signaling. Moreover, photo-activation of preBötC astrocytes is sufficient to elicit sigh activity, and this response is blocked with purinergic antagonists. We conclude that sighs are modulated through neuron-glia coupling in the preBötC network, where the distinct modulatory responses of neurons and glia allow for both rhythms to be independently regulated.
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spelling pubmed-104716082023-09-02 Purinergic signaling mediates neuroglial interactions to modulate sighs Severs, Liza J. Bush, Nicholas E. Quina, Lely A. Hidalgo-Andrade, Skyler Burgraff, Nicholas J. Dashevskiy, Tatiana Shih, Andy Y. Baertsch, Nathan A. Ramirez, Jan-Marino Nat Commun Article Sighs prevent the collapse of alveoli in the lungs, initiate arousal under hypoxic conditions, and are an expression of sadness and relief. Sighs are periodically superimposed on normal breaths, known as eupnea. Implicated in the generation of these rhythmic behaviors is the preBötzinger complex (preBötC). Our experimental evidence suggests that purinergic signaling is necessary to generate spontaneous and hypoxia-induced sighs in a mouse model. Our results demonstrate that driving calcium increases in astrocytes through pharmacological methods robustly increases sigh, but not eupnea, frequency. Calcium imaging of preBötC slices corroborates this finding with an increase in astrocytic calcium upon application of sigh modulators, increasing intracellular calcium through g-protein signaling. Moreover, photo-activation of preBötC astrocytes is sufficient to elicit sigh activity, and this response is blocked with purinergic antagonists. We conclude that sighs are modulated through neuron-glia coupling in the preBötC network, where the distinct modulatory responses of neurons and glia allow for both rhythms to be independently regulated. Nature Publishing Group UK 2023-08-31 /pmc/articles/PMC10471608/ /pubmed/37652903 http://dx.doi.org/10.1038/s41467-023-40812-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Severs, Liza J.
Bush, Nicholas E.
Quina, Lely A.
Hidalgo-Andrade, Skyler
Burgraff, Nicholas J.
Dashevskiy, Tatiana
Shih, Andy Y.
Baertsch, Nathan A.
Ramirez, Jan-Marino
Purinergic signaling mediates neuroglial interactions to modulate sighs
title Purinergic signaling mediates neuroglial interactions to modulate sighs
title_full Purinergic signaling mediates neuroglial interactions to modulate sighs
title_fullStr Purinergic signaling mediates neuroglial interactions to modulate sighs
title_full_unstemmed Purinergic signaling mediates neuroglial interactions to modulate sighs
title_short Purinergic signaling mediates neuroglial interactions to modulate sighs
title_sort purinergic signaling mediates neuroglial interactions to modulate sighs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10471608/
https://www.ncbi.nlm.nih.gov/pubmed/37652903
http://dx.doi.org/10.1038/s41467-023-40812-x
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