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Construction of a Set of Novel Transposon Vectors for Efficient Silencing of Protein and lncRNA Genes via CRISPR Interference

In recent years, CRISPR interference (CRISPRi) technology of gene silencing has emerged as a promising alternative to RNA interference (RNAi) surpassing the latter in terms of efficiency and accuracy. Here, we describe the construction of a set of transposon vectors suitable for constitutive or tetr...

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Detalles Bibliográficos
Autores principales: Czarnek, Maria, Kochan, Jakub, Wawro, Mateusz, Myrczek, Rafał, Bereta, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10471651/
https://www.ncbi.nlm.nih.gov/pubmed/36707469
http://dx.doi.org/10.1007/s12033-023-00675-5