R2C2+UMI: Combining concatemeric consensus sequencing with unique molecular identifiers enables ultra-accurate sequencing of amplicons on Oxford Nanopore Technologies sequencers

The sequencing of PCR amplicons is a core application of high-throughput sequencing technology. Using unique molecular identifiers (UMIs), individual amplified molecules can be sequenced to very high accuracy on an Illumina sequencer. However, Illumina sequencers have limited read length and are the...

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Detalles Bibliográficos
Autores principales: Deng, Dori Z.Q., Verhage, Jack, Neudorf, Celine, Corbett-Detig, Russell, Mekonen, Honey, Castaldi, Peter J., Vollmers, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473586/
https://www.ncbi.nlm.nih.gov/pubmed/37662385
http://dx.doi.org/10.1101/2023.08.19.553937

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473586/
https://www.ncbi.nlm.nih.gov/pubmed/37662385
http://dx.doi.org/10.1101/2023.08.19.553937

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