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Development of a sandwich ELISA for the specific quantitation of hemagglutinin (HA)-tagged proteins during their inducible expression in Escherichia coli

Heavy single-chain antibodies (VHH or nanobodies) are popular in the medical and analytical fields due to its small size, high solubility, stability, and other advantageous features. However, the usage of VHHs is limited by the low yield of its production and purification. In order to determine the...

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Detalles Bibliográficos
Autores principales: Yin, Zihan, He, Qiyi, Yang, Huiyi, Morisseau, Christophe, El-Sheikh, El-Sayed A., Li, Dongyang, Hammock, Bruce D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473979/
https://www.ncbi.nlm.nih.gov/pubmed/37505234
http://dx.doi.org/10.1007/s00216-023-04846-w