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Development of a sandwich ELISA for the specific quantitation of hemagglutinin (HA)-tagged proteins during their inducible expression in Escherichia coli
Heavy single-chain antibodies (VHH or nanobodies) are popular in the medical and analytical fields due to its small size, high solubility, stability, and other advantageous features. However, the usage of VHHs is limited by the low yield of its production and purification. In order to determine the...
Autores principales: | Yin, Zihan, He, Qiyi, Yang, Huiyi, Morisseau, Christophe, El-Sheikh, El-Sayed A., Li, Dongyang, Hammock, Bruce D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10473979/ https://www.ncbi.nlm.nih.gov/pubmed/37505234 http://dx.doi.org/10.1007/s00216-023-04846-w |
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