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Lactiplantibacillus plantarum as a novel platform for production and purification of integral membrane proteins using RseP as the benchmark

The present study describes a detailed procedure for expressing and purifying the integral membrane protein RseP using the pSIP system and Lactiplantibacillus plantarum as an expression host. RseP is a membrane-bound site-2-protease and a known antibacterial target in multiple human pathogens. In th...

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Detalles Bibliográficos
Autores principales: Kristensen, Sofie S., Lukassen, Marie V., Siebenhaar, Suzana, Diep, Dzung B., Morth, J. Preben, Mathiesen, Geir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474122/
https://www.ncbi.nlm.nih.gov/pubmed/37658186
http://dx.doi.org/10.1038/s41598-023-41559-7