Analysis of autophagy deficiency and cytotoxicity in autophagy-deficient human embryonic stem cell-derived neurons

Autophagy, a catabolic process governing cellular and energy homeostasis, is essential for cell survival and human health. Here, we present a protocol for generating autophagy-deficient (ATG5(−/−)) human neurons from human embryonic stem cell (hESC)-derived neural precursors. We describe steps for a...

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Detalles Bibliográficos
Autores principales: Korsgen, Miriam E., Sun, Congxin, Seranova, Elena, Zatyka, Malgorzata, Astuti, Dewi, Kataura, Tetsushi, Barrett, Timothy, Korolchuk, Viktor I., Sarkar, Sovan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474488/
https://www.ncbi.nlm.nih.gov/pubmed/37624702
http://dx.doi.org/10.1016/j.xpro.2023.102529

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474488/
https://www.ncbi.nlm.nih.gov/pubmed/37624702
http://dx.doi.org/10.1016/j.xpro.2023.102529

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