Cargando…

Analysis of autophagy deficiency and cytotoxicity in autophagy-deficient human embryonic stem cell-derived neurons

Autophagy, a catabolic process governing cellular and energy homeostasis, is essential for cell survival and human health. Here, we present a protocol for generating autophagy-deficient (ATG5(−/−)) human neurons from human embryonic stem cell (hESC)-derived neural precursors. We describe steps for a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Korsgen, Miriam E., Sun, Congxin, Seranova, Elena, Zatyka, Malgorzata, Astuti, Dewi, Kataura, Tetsushi, Barrett, Timothy, Korolchuk, Viktor I., Sarkar, Sovan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474488/ https://www.ncbi.nlm.nih.gov/pubmed/37624702 http://dx.doi.org/10.1016/j.xpro.2023.102529

Ejemplares similares

Dysregulation of autophagy as a common mechanism in lysosomal storage diseases
por: Seranova, Elena, et al.
Publicado: (2017)

Autophagy in Rare (NonLysosomal) Neurodegenerative Diseases
por: Zatyka, Malgorzata, et al.
Publicado: (2020)

Selective Autophagy and Xenophagy in Infection and Disease
por: Sharma, Vartika, et al.
Publicado: (2018)

Biomedical Implications of Autophagy in Macromolecule Storage Disorders
por: Palhegyi, Adina Maria, et al.
Publicado: (2019)

The pROS of Autophagy in Neuronal Health
por: Sedlackova, Lucia, et al.
Publicado: (2020)

Depletion of WFS1 compromises mitochondrial function in hiPSC-derived neuronal models of Wolfram syndrome
por: Zatyka, Malgorzata, et al.
Publicado: (2023)

Laforin, the most common protein mutated in Lafora disease, regulates autophagy
por: Aguado, Carmen, et al.
Publicado: (2010)

Autophagy-monitoring and autophagy-deficient mice
por: Kuma, Akiko, et al.
Publicado: (2017)

Chemical Screening Approaches Enabling Drug Discovery of Autophagy Modulators for Biomedical Applications in Human Diseases
por: Panda, Prashanta Kumar, et al.
Publicado: (2019)

Complex Inhibitory Effects of Nitric Oxide on Autophagy
por: Sarkar, Sovan, et al.
Publicado: (2011)

Dysregulation of mTORC1/autophagy axis in senescence
por: Carroll, Bernadette, et al.
Publicado: (2017)

The crosstalk of NAD, ROS and autophagy in cellular health and ageing
por: Sedlackova, Lucia, et al.
Publicado: (2020)

Autophagy deficiency in neurodevelopmental disorders
por: Deng, Zhiqiang, et al.
Publicado: (2021)

mTORC1 as the main gateway to autophagy
por: Rabanal-Ruiz, Yoana, et al.
Publicado: (2017)

Deletion of the Huntingtin Polyglutamine Stretch Enhances Neuronal Autophagy and Longevity in Mice
por: Zheng, Shuqiu, et al.
Publicado: (2010)

Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P(2)
por: Ferguson, Cole J., et al.
Publicado: (2009)

REST/NRSF deficiency impairs autophagy and leads to cellular senescence in neurons
por: Rocchi, Anna, et al.
Publicado: (2021)

Antioxidants can inhibit basal autophagy and enhance neurodegeneration in models of polyglutamine disease
por: Underwood, Benjamin R., et al.
Publicado: (2010)

Nutrition and autophagy deficiency in critical illness
por: Vanhorebeek, Ilse, et al.
Publicado: (2023)

Editorial: Autophagy: From Big Data to Physiological Significance
por: Sarkar, Sovan, et al.
Publicado: (2020)

Corrigendum to: REST/NRSF deficiency impairs autophagy and leads to cellular senescence in neurons
Publicado: (2023)

Autophagy Inhibition Compromises Degradation of Ubiquitin-Proteasome Pathway Substrates
por: Korolchuk, Viktor I., et al.
Publicado: (2009)

Oxidation of p62 as an evolutionary adaptation to promote autophagy in stress conditions
por: Otten, Elsje G., et al.
Publicado: (2018)

Zinc Deficiency Induces Autophagy in HT-22 Mouse Hippocampal Neuronal Cell Line
por: Kim, Si-Yeon, et al.
Publicado: (2022)

Chemical Inducers of Autophagy That Enhance the Clearance of Mutant Proteins in Neurodegenerative Diseases
por: Renna, Maurizio, et al.
Publicado: (2010)

The mTORC1-autophagy pathway is a target for senescent cell elimination
por: Kucheryavenko, Olena, et al.
Publicado: (2019)

FBXL4 deficiency increases mitochondrial removal by autophagy
por: Alsina, David, et al.
Publicado: (2020)

Deficient Chaperone-Mediated Autophagy Promotes Inflammation and Atherosclerosis
por: Qiao, Lei, et al.
Publicado: (2021)

Linking of Senescence to Autophagy Deficiency in Chronic Liver Disease
por: Ma, Xiaowen, et al.
Publicado: (2022)

T-cell Intracellular Antigen (TIA)-Proteins Deficiency in Murine Embryonic Fibroblasts Alters Cell Cycle Progression and Induces Autophagy
por: Sánchez-Jiménez, Carmen, et al.
Publicado: (2013)

Lithium induces autophagy by inhibiting inositol monophosphatase
por: Sarkar, Sovan, et al.
Publicado: (2005)

SORL1 deficiency in human excitatory neurons causes APP-dependent defects in the endolysosome-autophagy network
por: Hung, Christy, et al.
Publicado: (2021)

Cardiac glycosides restore autophagy flux in an iPSC-derived neuronal model of WDR45 deficiency
por: Papandreou, Apostolos, et al.
Publicado: (2023)

LC3-positive structures are prominent in autophagy-deficient cells
por: Runwal, Gautam, et al.
Publicado: (2019)

PTEN deficiency permits the formation of pancreatic cancer in the absence of autophagy
por: Rosenfeldt, Mathias T, et al.
Publicado: (2017)

Progranulin deficiency causes impairment of autophagy and TDP-43 accumulation
por: Chang, Michael C., et al.
Publicado: (2017)

Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice
por: Hoffmann-Conaway, Sheila, et al.
Publicado: (2020)

Investigating AKT activation and autophagy in immunoproteasome-deficient retinal cells
por: Karim, Md. Razaul, et al.
Publicado: (2020)

Deficient Chaperone-Mediated Autophagy Promotes Lipid Accumulation in Macrophage
por: Qiao, Lei, et al.
Publicado: (2020)

Senescence Connects Autophagy Deficiency to Inflammation and Tumor Progression in the Liver
por: Huda, Nazmul, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_po6sbsbkuq3r9junms58t8b2u5