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Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines
IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profil...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475480/ https://www.ncbi.nlm.nih.gov/pubmed/37670790 http://dx.doi.org/10.1016/j.isci.2023.107619 |
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author | Van Coillie, Julie Pongracz, Tamas Šuštić, Tonći Wang, Wenjun Nouta, Jan Le Gars, Mathieu Keijzer, Sofie Linty, Federica Cristianawati, Olvi Keijser, Jim B.D. Visser, Remco van Vught, Lonneke A. Slim, Marleen A. van Mourik, Niels Smit, Merel J. Sander, Adam Schmidt, David E. Steenhuis, Maurice Rispens, Theo Nielsen, Morten A. Mordmüller, Benjamin G. Vlaar, Alexander P.J. Ellen van der Schoot, C. Roozendaal, Ramon Wuhrer, Manfred Vidarsson, Gestur |
author_facet | Van Coillie, Julie Pongracz, Tamas Šuštić, Tonći Wang, Wenjun Nouta, Jan Le Gars, Mathieu Keijzer, Sofie Linty, Federica Cristianawati, Olvi Keijser, Jim B.D. Visser, Remco van Vught, Lonneke A. Slim, Marleen A. van Mourik, Niels Smit, Merel J. Sander, Adam Schmidt, David E. Steenhuis, Maurice Rispens, Theo Nielsen, Morten A. Mordmüller, Benjamin G. Vlaar, Alexander P.J. Ellen van der Schoot, C. Roozendaal, Ramon Wuhrer, Manfred Vidarsson, Gestur |
author_sort | Van Coillie, Julie |
collection | PubMed |
description | IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps. |
format | Online Article Text |
id | pubmed-10475480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104754802023-09-05 Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines Van Coillie, Julie Pongracz, Tamas Šuštić, Tonći Wang, Wenjun Nouta, Jan Le Gars, Mathieu Keijzer, Sofie Linty, Federica Cristianawati, Olvi Keijser, Jim B.D. Visser, Remco van Vught, Lonneke A. Slim, Marleen A. van Mourik, Niels Smit, Merel J. Sander, Adam Schmidt, David E. Steenhuis, Maurice Rispens, Theo Nielsen, Morten A. Mordmüller, Benjamin G. Vlaar, Alexander P.J. Ellen van der Schoot, C. Roozendaal, Ramon Wuhrer, Manfred Vidarsson, Gestur iScience Article IgG antibodies are important mediators of vaccine-induced immunity through complement- and Fc receptor-dependent effector functions. Both are influenced by the composition of the conserved N-linked glycan located in the IgG Fc domain. Here, we compared the anti-Spike (S) IgG1 Fc glycosylation profiles in response to mRNA, adenoviral, and protein-based COVID-19 vaccines by mass spectrometry (MS). All vaccines induced a transient increase of antigen-specific IgG1 Fc galactosylation and sialylation. An initial, transient increase of afucosylated IgG was induced by membrane-encoding S protein formulations. A fucose-sensitive ELISA for antigen-specific IgG (FEASI) exploiting FcγRIIIa affinity for afucosylated IgG was used as an orthogonal method to confirm the LC-MS-based afucosylation readout. Our data suggest that vaccine-induced anti-S IgG glycosylation is dynamic, and although variation is seen between different vaccine platforms and individuals, the evolution of glycosylation patterns display marked overlaps. Elsevier 2023-08-14 /pmc/articles/PMC10475480/ /pubmed/37670790 http://dx.doi.org/10.1016/j.isci.2023.107619 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Van Coillie, Julie Pongracz, Tamas Šuštić, Tonći Wang, Wenjun Nouta, Jan Le Gars, Mathieu Keijzer, Sofie Linty, Federica Cristianawati, Olvi Keijser, Jim B.D. Visser, Remco van Vught, Lonneke A. Slim, Marleen A. van Mourik, Niels Smit, Merel J. Sander, Adam Schmidt, David E. Steenhuis, Maurice Rispens, Theo Nielsen, Morten A. Mordmüller, Benjamin G. Vlaar, Alexander P.J. Ellen van der Schoot, C. Roozendaal, Ramon Wuhrer, Manfred Vidarsson, Gestur Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines |
title | Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines |
title_full | Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines |
title_fullStr | Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines |
title_full_unstemmed | Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines |
title_short | Comparative analysis of spike-specific IgG Fc glycoprofiles elicited by adenoviral, mRNA, and protein-based SARS-CoV-2 vaccines |
title_sort | comparative analysis of spike-specific igg fc glycoprofiles elicited by adenoviral, mrna, and protein-based sars-cov-2 vaccines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475480/ https://www.ncbi.nlm.nih.gov/pubmed/37670790 http://dx.doi.org/10.1016/j.isci.2023.107619 |
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