An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly

Golden Gate Assembly is an efficient and rapid cloning method but requires dedicated vectors. Here, we modified Golden Gate to expand its compatibility to a broader range of destination vectors while maintaining its strengths. Our Expanded Golden Gate (ExGG) assembly adds to the insert(s) type IIS r...

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Detalles Bibliográficos
Autores principales: Sorida, Masato, Bonasio, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475842/
https://www.ncbi.nlm.nih.gov/pubmed/37671021
http://dx.doi.org/10.1016/j.crmeth.2023.100564

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475842/
https://www.ncbi.nlm.nih.gov/pubmed/37671021
http://dx.doi.org/10.1016/j.crmeth.2023.100564

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