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An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly
Golden Gate Assembly is an efficient and rapid cloning method but requires dedicated vectors. Here, we modified Golden Gate to expand its compatibility to a broader range of destination vectors while maintaining its strengths. Our Expanded Golden Gate (ExGG) assembly adds to the insert(s) type IIS r...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475842/ https://www.ncbi.nlm.nih.gov/pubmed/37671021 http://dx.doi.org/10.1016/j.crmeth.2023.100564 |