Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas

CRISPR-Cas genome engineering in the unicellular green algal model Chlamydomonas reinhardtii has until now been primarily applied to targeted gene disruption, whereas scarless knockin transgenesis has generally been considered difficult in practice. We have developed an efficient homology-directed m...

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Detalles Bibliográficos
Autores principales: Nievergelt, Adrian Pascal, Diener, Dennis Ray, Bogdanova, Aliona, Brown, Thomas, Pigino, Gaia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475843/
https://www.ncbi.nlm.nih.gov/pubmed/37671018
http://dx.doi.org/10.1016/j.crmeth.2023.100562

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10475843/
https://www.ncbi.nlm.nih.gov/pubmed/37671018
http://dx.doi.org/10.1016/j.crmeth.2023.100562

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