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Development of a versatile LCM-Seq method for spatial transcriptomics of fluorescently tagged cholinergic neuron populations

Single-cell transcriptomics are powerful tools to define neuronal cell types based on co-expressed gene clusters. Limited RNA input in these technologies necessarily compromises transcriptome coverage and accuracy of differential expression analysis. We propose that bulk RNA-Seq of neuronal pools de...

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Detalles Bibliográficos
Autores principales: Rumpler, Éva, Göcz, Balázs, Skrapits, Katalin, Sárvári, Miklós, Takács, Szabolcs, Farkas, Imre, Póliska, Szilárd, Papp, Márton, Solymosi, Norbert, Hrabovszky, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477691/
https://www.ncbi.nlm.nih.gov/pubmed/37536628
http://dx.doi.org/10.1016/j.jbc.2023.105121