Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells

Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27(−) γδ T cells, and ATP delivery. We then deta...

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Detalles Bibliográficos
Autores principales: Wang, Yunke, Yan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477735/
https://www.ncbi.nlm.nih.gov/pubmed/37632744
http://dx.doi.org/10.1016/j.xpro.2023.102532

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477735/
https://www.ncbi.nlm.nih.gov/pubmed/37632744
http://dx.doi.org/10.1016/j.xpro.2023.102532

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