Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells

Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27(−) γδ T cells, and ATP delivery. We then deta...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yunke, Yan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477735/
https://www.ncbi.nlm.nih.gov/pubmed/37632744
http://dx.doi.org/10.1016/j.xpro.2023.102532
Descripción
Sumario:Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27(−) γδ T cells, and ATP delivery. We then detail functional evaluation of γδ T cells by flow cytometry. Appropriate concentrations of control and ATP vesicles are detailed for intracellular ATP delivery, which can also be applied to other immune cells. For complete details on the use and execution of this protocol, please refer to Wang et al. (2023).(1)

Ejemplares similares