Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells

Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27(−) γδ T cells, and ATP delivery. We then deta...

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Detalles Bibliográficos
Autores principales: Wang, Yunke, Yan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477735/
https://www.ncbi.nlm.nih.gov/pubmed/37632744
http://dx.doi.org/10.1016/j.xpro.2023.102532
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author Wang, Yunke
Yan, Jun
author_facet Wang, Yunke
Yan, Jun
author_sort Wang, Yunke
collection PubMed
description Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27(−) γδ T cells, and ATP delivery. We then detail functional evaluation of γδ T cells by flow cytometry. Appropriate concentrations of control and ATP vesicles are detailed for intracellular ATP delivery, which can also be applied to other immune cells. For complete details on the use and execution of this protocol, please refer to Wang et al. (2023).(1)
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spelling pubmed-104777352023-09-06 Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells Wang, Yunke Yan, Jun STAR Protoc Protocol Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27(−) γδ T cells, and ATP delivery. We then detail functional evaluation of γδ T cells by flow cytometry. Appropriate concentrations of control and ATP vesicles are detailed for intracellular ATP delivery, which can also be applied to other immune cells. For complete details on the use and execution of this protocol, please refer to Wang et al. (2023).(1) Elsevier 2023-08-25 /pmc/articles/PMC10477735/ /pubmed/37632744 http://dx.doi.org/10.1016/j.xpro.2023.102532 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wang, Yunke
Yan, Jun
Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells
title Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells
title_full Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells
title_fullStr Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells
title_full_unstemmed Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells
title_short Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells
title_sort intracellular atp delivery to in vitro expanded mouse cd27(−) γδ t cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477735/
https://www.ncbi.nlm.nih.gov/pubmed/37632744
http://dx.doi.org/10.1016/j.xpro.2023.102532
work_keys_str_mv AT wangyunke intracellularatpdeliverytoinvitroexpandedmousecd27gdtcells
AT yanjun intracellularatpdeliverytoinvitroexpandedmousecd27gdtcells

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