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Intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells

Intracellular ATP supports the function of γδT17 cells in mice. Here, we present a protocol for intracellular ATP delivery to in vitro expanded mouse CD27(−) γδ T cells. We describe steps for pre-coating well plates, preparing lymphocytes, culturing CD27(−) γδ T cells, and ATP delivery. We then deta...

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Detalles Bibliográficos
Autores principales:	Wang, Yunke, Yan, Jun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477735/ https://www.ncbi.nlm.nih.gov/pubmed/37632744 http://dx.doi.org/10.1016/j.xpro.2023.102532

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