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Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets
Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated frac...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477737/ https://www.ncbi.nlm.nih.gov/pubmed/37632746 http://dx.doi.org/10.1016/j.xpro.2023.102528 |