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Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets

Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated frac...

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Detalles Bibliográficos
Autores principales: Seigneurin-Berny, Daphné, Touat-Todeschini, Leila, Timcheva, Kalina, Verdel, André
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477737/
https://www.ncbi.nlm.nih.gov/pubmed/37632746
http://dx.doi.org/10.1016/j.xpro.2023.102528