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Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets
Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated frac...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477737/ https://www.ncbi.nlm.nih.gov/pubmed/37632746 http://dx.doi.org/10.1016/j.xpro.2023.102528 |
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author | Seigneurin-Berny, Daphné Touat-Todeschini, Leila Timcheva, Kalina Verdel, André |
author_facet | Seigneurin-Berny, Daphné Touat-Todeschini, Leila Timcheva, Kalina Verdel, André |
author_sort | Seigneurin-Berny, Daphné |
collection | PubMed |
description | Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets. This protocol can be adapted to other human or mammalian chromatin-associated RNA binding proteins. For complete details on the use and execution of this protocol, please refer to Timcheva et al.(1) |
format | Online Article Text |
id | pubmed-10477737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104777372023-09-06 Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets Seigneurin-Berny, Daphné Touat-Todeschini, Leila Timcheva, Kalina Verdel, André STAR Protoc Protocol Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets. This protocol can be adapted to other human or mammalian chromatin-associated RNA binding proteins. For complete details on the use and execution of this protocol, please refer to Timcheva et al.(1) Elsevier 2023-08-25 /pmc/articles/PMC10477737/ /pubmed/37632746 http://dx.doi.org/10.1016/j.xpro.2023.102528 Text en © 2023 The Authors. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Seigneurin-Berny, Daphné Touat-Todeschini, Leila Timcheva, Kalina Verdel, André Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets |
title | Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets |
title_full | Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets |
title_fullStr | Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets |
title_full_unstemmed | Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets |
title_short | Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets |
title_sort | protocol to study the role of a human nuclear m6a rna reader on chromatin-associated rna targets |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10477737/ https://www.ncbi.nlm.nih.gov/pubmed/37632746 http://dx.doi.org/10.1016/j.xpro.2023.102528 |
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