Shortened CRISPR-Cas9 arrays enable multiplexed gene targeting in bacteria from a smaller DNA footprint

CRISPR technologies comprising a Cas nuclease and a guide RNA (gRNA) can utilize multiple gRNAs to enact multi-site editing or regulation in the same cell. Nature devised a highly compact means of encoding gRNAs in the form of CRISPR arrays composed of conserved repeats separated by targeting spacer...

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Detalles Bibliográficos
Autores principales: Gawlitt, Sandra, Liao, Chunyu, Achmedov, Tatjana, Beisel, Chase L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10478742/
https://www.ncbi.nlm.nih.gov/pubmed/37654098
http://dx.doi.org/10.1080/15476286.2023.2247247

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10478742/
https://www.ncbi.nlm.nih.gov/pubmed/37654098
http://dx.doi.org/10.1080/15476286.2023.2247247

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