DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging

Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporation of the DNA-based signal amplification by e...

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Autores principales: Hosogane, Tsuyoshi, Casanova, Ruben, Bodenmiller, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482679/
https://www.ncbi.nlm.nih.gov/pubmed/37653118
http://dx.doi.org/10.1038/s41592-023-01976-y
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author Hosogane, Tsuyoshi
Casanova, Ruben
Bodenmiller, Bernd
author_facet Hosogane, Tsuyoshi
Casanova, Ruben
Bodenmiller, Bernd
author_sort Hosogane, Tsuyoshi
collection PubMed
description Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporation of the DNA-based signal amplification by exchange reaction, immuno-SABER. We applied SABER-IMC to image the tumor immune microenvironment in human melanoma by simultaneous imaging of 18 markers with immuno-SABER and 20 markers without amplification. SABER-IMC enabled the identification of immune cell phenotypic markers, such as T cell co-receptors and their ligands, that are not detectable with IMC.
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spelling pubmed-104826792023-09-08 DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging Hosogane, Tsuyoshi Casanova, Ruben Bodenmiller, Bernd Nat Methods Brief Communication Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporation of the DNA-based signal amplification by exchange reaction, immuno-SABER. We applied SABER-IMC to image the tumor immune microenvironment in human melanoma by simultaneous imaging of 18 markers with immuno-SABER and 20 markers without amplification. SABER-IMC enabled the identification of immune cell phenotypic markers, such as T cell co-receptors and their ligands, that are not detectable with IMC. Nature Publishing Group US 2023-08-31 2023 /pmc/articles/PMC10482679/ /pubmed/37653118 http://dx.doi.org/10.1038/s41592-023-01976-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Communication
Hosogane, Tsuyoshi
Casanova, Ruben
Bodenmiller, Bernd
DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
title DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
title_full DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
title_fullStr DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
title_full_unstemmed DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
title_short DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
title_sort dna-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482679/
https://www.ncbi.nlm.nih.gov/pubmed/37653118
http://dx.doi.org/10.1038/s41592-023-01976-y
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