Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Prime editing enables a wide variety of precise genome edits in living cells. Here we use protein evolution and engineering to generate prime editors with reduced size and improved efficiency. Using phage-assisted evolution, we improved editing efficiencies of compact reverse transcriptases by up to...

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Detalles Bibliográficos
Autores principales: Doman, Jordan L., Pandey, Smriti, Neugebauer, Monica E., An, Meirui, Davis, Jessie R., Randolph, Peyton B., McElroy, Amber, Gao, Xin D., Raguram, Aditya, Richter, Michelle F., Everette, Kelcee A., Banskota, Samagya, Tian, Kathryn, Tao, Y. Allen, Tolar, Jakub, Osborn, Mark J., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2023
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482982/
https://www.ncbi.nlm.nih.gov/pubmed/37657419
http://dx.doi.org/10.1016/j.cell.2023.07.039

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10482982/
https://www.ncbi.nlm.nih.gov/pubmed/37657419
http://dx.doi.org/10.1016/j.cell.2023.07.039

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