Cargando…

Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber

[Image: see text] The fluorescence attenuation caused by the absorption of the excitation and/or emission light is called the Inner Filter Effect (IFE) and can lead to a nonlinear fluorescence concentration response. In this article, we propose the AddAbs (Added Absorber) method, which counterintuit...

Descripción completa

Detalles Bibliográficos
Autores principales: Friganović, Tomislav, Weitner, Tin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10483458/
https://www.ncbi.nlm.nih.gov/pubmed/37606558
http://dx.doi.org/10.1021/acs.analchem.3c01295