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Reducing the Inner Filter Effect in Microplates by Increasing Absorbance? Linear Fluorescence in Highly Concentrated Fluorophore Solutions in the Presence of an Added Absorber
[Image: see text] The fluorescence attenuation caused by the absorption of the excitation and/or emission light is called the Inner Filter Effect (IFE) and can lead to a nonlinear fluorescence concentration response. In this article, we propose the AddAbs (Added Absorber) method, which counterintuit...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10483458/ https://www.ncbi.nlm.nih.gov/pubmed/37606558 http://dx.doi.org/10.1021/acs.analchem.3c01295 |