The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice

CRISPR/Cas9-based genome editing represents an unprecedented potential for plant breeding. Unlike animal cells, plant cells contain a rigid cell wall, genome editing tool delivery into plant cells is thus challenging. In particular, the delivery of the Cas9-gRNA ribonucleoprotein (RNP) into plant ce...

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Detalles Bibliográficos
Autores principales: Nakamura, Akiyoshi, Yano, Tsubasa, Mitsuda, Nobutaka, Furubayashi, Maiko, Ito, Seiichiro, Sugano, Shigeo S., Terakawa, Teruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484913/
https://www.ncbi.nlm.nih.gov/pubmed/37679413
http://dx.doi.org/10.1038/s41598-023-40433-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484913/
https://www.ncbi.nlm.nih.gov/pubmed/37679413
http://dx.doi.org/10.1038/s41598-023-40433-w

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