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The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice
CRISPR/Cas9-based genome editing represents an unprecedented potential for plant breeding. Unlike animal cells, plant cells contain a rigid cell wall, genome editing tool delivery into plant cells is thus challenging. In particular, the delivery of the Cas9-gRNA ribonucleoprotein (RNP) into plant ce...
Autores principales: | Nakamura, Akiyoshi, Yano, Tsubasa, Mitsuda, Nobutaka, Furubayashi, Maiko, Ito, Seiichiro, Sugano, Shigeo S., Terakawa, Teruhiko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484913/ https://www.ncbi.nlm.nih.gov/pubmed/37679413 http://dx.doi.org/10.1038/s41598-023-40433-w |
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