Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sorting

BCAM-positive basal limbal epithelial cells are an early transit-amplifying cell population (TAC) capable of holoclone formation and corneal epithelial differentiation. Here, we present a protocol for isolating BCAM-positive cells from human donor corneas by flow cytometry and cell sorting. We descr...

Descripción completa

Detalles Bibliográficos
Autores principales: Sasamoto, Yuzuru, Yeung, Philip C., Tran, Johnathan, Frank, Markus H., Frank, Natasha Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485628/
https://www.ncbi.nlm.nih.gov/pubmed/37669162
http://dx.doi.org/10.1016/j.xpro.2023.102503

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485628/
https://www.ncbi.nlm.nih.gov/pubmed/37669162
http://dx.doi.org/10.1016/j.xpro.2023.102503

Ejemplares similares