Quantification of total and phosphorylated STAT3 by calibrated western blotting

Quantification of intracellular proteins is essential to understand signaling. Here, we describe quantification of the expression and phosphorylation of the transcription factor STAT3. We present isolation of total and phosphorylated STAT3 from cell lysates by immunoprecipitation, followed by SDS-PA...

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Detalles Bibliográficos
Autores principales: Köhler, Nadine, Miri, Niloufarsadat, Dittrich, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485629/
https://www.ncbi.nlm.nih.gov/pubmed/37669163
http://dx.doi.org/10.1016/j.xpro.2023.102508

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485629/
https://www.ncbi.nlm.nih.gov/pubmed/37669163
http://dx.doi.org/10.1016/j.xpro.2023.102508

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